Experiment information
Accession CRX050790
Organism Oryza sativa Indica Group
Title WT-R1
BioProject PRJCA001490
BioSample SAMC071052
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted from the fully unfolded leaf blades located at the same position on the main stems of 40-day-old plants using RNeasy Plant Mini Kit (Qiagen), according to the manufacturer’s instructions. The concentration and quality of total RNA were evaluated using a NanoDrop spectrophotometer and Agilent 2100 Bioanalyzer, respectively. Libraries for RNA-Seq analysis were constructed from 3 μg of total RNA per sample using NEBNext®Ultra™ RNA Library Prep Kit for Illumina (NEB), according to the manufacturer’s recommendations. Each sample was then labeled with a unique index code. Cluster generation was performed using TruSeq PE Cluster Kit v4-cBot-HS (Illumina). After cluster generation, the libraries were sequenced using the Illumina HiSeq 2500 platform (Berry Genomics) to generate paired-end reads. RNA-Seq TRANSCRIPTOMIC PolyA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 280
Planned read length (bp): 150
Release date2019-12-01
Run
Run accession Release date Run data file information
File nameFile size (MB)
CRR056361 2019-12-01 WT-R1_L4_366366.R1.clean.fastq.gz
WT-R1_L4_366366.R2.clean.fastq.gz
3,079.96
3,245.95
Submitterhao li lin (lihaoshzu@163.com)
OrganizationsShihezi university
Date submitted2019-06-09
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