Experiment information
Accession CRX050726
Organism Populus tomentosa
Title 48 hpi -3
BioProject PRJCA001484
BioSample SAMC070961
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was isolated using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the instruction manual. The quality of RNA were fist determined by agarose gel electrophoresis to ensure that clear bands were visible. The RNA integrity number (RIN) of all samples were also determined using a NanoDrop 2000 sectrophotometer (Thermo Scientific, Vilnius, Lithuania). Samples with sufficient quality were sent for RNA-seq complementary DNA (cDNA) library construction and submitted to an Illumina HiSeq 4000 (Illumina, Inc., San Diego, CA, USA) sequencing platform sequenced on a flow cell . RNA-Seq TRANSCRIPTOMIC cDNA PAIRED
Processing Planned read length (bp) for mate 1: 300
Planned read length (bp) for mate 2: 300
Insert size (bp): 150
Planned read length (bp): 250
Release date2019-10-10
Run
Run accession Release date Run data file information
File nameFile size (MB)
CRR056822 2019-10-10 s2d_3_1.1.clean.fq.gz
s2d_3_1.2.clean.fq.gz
1,523.21
1,587.64
Submittersisi Chen (sisichen@bjfu.edu.cn)
OrganizationsBeijing Forestry University
Date submitted2019-06-04