Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
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Total RNA was isolated using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the instruction manual. The quality of RNA were fist determined by agarose gel electrophoresis to ensure that clear bands were visible. The RNA integrity number (RIN) of all samples were also determined using a NanoDrop 2000 sectrophotometer (Thermo Scientific, Vilnius, Lithuania). Samples with sufficient quality were sent for RNA-seq complementary DNA (cDNA) library construction and submitted to an Illumina HiSeq 4000 (Illumina, Inc., San Diego, CA, USA) sequencing platform sequenced on a flow cell . |
RNA-Seq |
TRANSCRIPTOMIC |
cDNA |
PAIRED
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