Experiment information
Accession CRX050713
Organism Mus
Title ChIP H3K27me3-control 1
BioProject PRJCA001414
BioSample SAMC070947
Platform BGISEQ-500
Library
Library name Construction protocol Strategy Source Selection Layout
ChIP was performed according to Agilent Mammalian ChIP-on-chip manual. Briefly, 1x108 ES cells were fixed with 1% formaldehyde for 10 minutes at room temperature. Then the reactions were stopped by 0.125M glycine for 5 min. Chromatin were sonicated to an average of 200-500bp (ChIP-Seq) or 500bp-1000bp (ChIP-qPCR) using Covaris Sonication System (USA) according to the manual. Then 1% Triton X-100 was added to the sonicated chromatin solutions to a final 0.1% Triton X-100. After centrifuge, 50 ul of supernatant were saved as input. The rest of chromatin solution was incubated with Dynabeads previously coupled with ChIP grade antibodies overnight at 4℃ with rotation. After extensive washes with washing buffer, the complexes were reverse cross-linked overnight at 65℃. DNAs were extracted by hydroxybenzene-chloroform-Isoamyl alcohol and purified by Phase Lock Gel (Tiangen, China). The ChIPed DNA were used for library constructions and deep sequencing by Shenzhen BGI (Wuhan, China). For ChIP-PCR, ChIPed DNA were quantified by quantitative real-time PCR(qPCR). The enrichment was calculated relative to the amount of input chromatin. ChIP-Seq GENOMIC unspecified FRAGMENT
Processing
Release date2019-12-21
Run
Run accession Release date Run data file information
File nameFile size (MB)
CRR056289 2019-12-21 Clean_CL100100938_L01_524.fq.gz 1,028.28
SubmitterSun Xiaoyun (sunnyxyun@163.com)
Organizations Institute of Hydrobiology, Chinese Academy of Sciences
Date submitted2019-06-04