Experiment information
Accession CRX050204
Organism Oryza sativa Japonica Group
Title Nipponbare.HiC
BioProject PRJCA001420
BioSample SAMC069969
Platform Illumina HiSeq X Ten
Library name Construction protocol Strategy Source Selection Layout
Briefly, the leaves were fixed with formaldehyde, lysed, and then the cross-linked DNA digested with MboI over night. Sticky ends were biotinylated and proximity-ligated to form chimeric junctions, that were enriched for and then physically sheared to a size of 500-700 bp. Chimeric fragments representing the original cross-linked long-distance physical interactions were then processed into paired-end sequencing libraries and 150 bp paired-end reads were produced on Illumina HiSeq X platform. Hi-C GENOMIC Restriction Digest PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 500
Release date2019-05-31
Run accession Release date Run data file information
File nameFile size (MB)
CRR055774 2019-05-31 Nipponbare.HiC_R1.fastq.gz
SubmitterJing lin (lolyemily@163.com)
OrganizationsFujian Agriculture and Forestry University
Date submitted2019-05-14
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