Experiment information
Accession CRX050066
Organism Bos grunniens x Bos taurus
Title RNA-seq:DTE2
BioProject PRJCA001418
BioSample SAMC069847
Platform Illumina HiSeq 2500
Library name Construction protocol Strategy Source Selection Layout
Total RNA from the frozen samples was extracted using the TRIzol reagent (Invitrogen, USA) following the manufacturer’s protocol. Genomic DNA contamination was first removed using RNA-free DNase I, and the RNA integrity and quality were then analyzed using a Bioanalyzer (Agilent, USA). The RNA integrity threshold was RIN ? 6.8. PolyA(+) RNA-seq libraries were constructed using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s recommendations. The resulting cDNA was first cleaved into 300-500-bp fragments to construct libraries according to the manufacturer’s instructions, and the libraries were then sequenced using the Illumina HiSeq 2000/2500 platform RNA-Seq TRANSCRIPTOMIC PolyA PAIRED
Processing Planned read length (bp) for mate 1: 125
Planned read length (bp) for mate 2: 125
Insert size (bp): 150
Release date2019-06-01
Run accession Release date Run data file information
File nameFile size (MB)
CRR055638 2019-06-01 PO4.R1.clean.fastq.gz
SubmitterChen Weihuang (chenweiihuang@163.com)
OrganizationsNorthwest A&F University
Date submitted2019-05-10