Experiment information
Accession CRX050038
Organism Equus caballus x Equus asinus
Title RNA-seq:HM4
BioProject PRJCA001418
BioSample SAMC069819
Platform Illumina HiSeq 2000
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA from the frozen samples was extracted using the TRIzol reagent (Invitrogen, USA) following the manufacturer’s protocol. Genomic DNA contamination was first removed using RNA-free DNase I, and the RNA integrity and quality were then analyzed using a Bioanalyzer (Agilent, USA). The RNA integrity threshold was RIN ? 6.8. PolyA(+) RNA-seq libraries were constructed using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s recommendations. The resulting cDNA was first cleaved into 300-500-bp fragments to construct libraries according to the manufacturer’s instructions, and the libraries were then sequenced using the Illumina HiSeq 2000/2500 platform RNA-Seq TRANSCRIPTOMIC PolyA PAIRED
Processing Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
Insert size (bp): 150
Release date2019-06-01
Run
Run accession Run data file information
File nameFile size (MB)
CRR055610 CRR055610_f1.fq.gz
CRR055610_r2.fq.gz
2,195.49
2,179.93
SubmitterChen Weihuang (chenweiihuang@163.com)
OrganizationNorthwest A&F University
Date submitted2019-05-10