Experiment information
Accession CRX049658
Organism Ailuropoda melanoleuca
Title NN1301
BioProject PRJCA000366
BioSample SAMC011124
Platform Illumina Genome Analyzer
Library
Library name Construction protocol Strategy Source Selection Layout
DNA of sorted NCSCs was extracted from the cell line using a QIAamp DNA Mini Kit, sheared to approximately 300-500 bp using a Covaris S220 instrument. Then the libraries were constructed through end-repair, A-tailing, adapter ligation and bisulfate-converted using a ZymoEZ DNA Methylation Kit. Then it was sequenced on Illummia Hiseq 2000. WGS METAGENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
Insert size (bp): 500
Release date2019-05-13
Run
Run accession Run data file information
File nameFile size (MB)
CRR055214 CRR055214_f1.fq.gz
CRR055214_r2.fq.gz
3,283.99
3,216.99
SubmitterYun Ding (yun_ding@qq.com)
OrganizationInstitute of Zoology, Chinese Academy of Sciences
Date submitted2019-05-08