Accession | CRX049641 |
Organism | Camellia sinensis var. assamica |
Title | Tea tree (Camellia sinensis var. assamica) whole genome sequencing of library_2 |
BioProject | PRJCA001416 |
BioSample | SAMC069442 |
Platform | Illumina HiSeq 2000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
High-quality genomic DNA was extracted from leaves using a modified CTAB method20. RNase A and proteinase K were separately used to remove RNA and protein contamination. The quality and quantity of the isolated DNA were separately checked by electrophoresis on a 0.8% agarose gel and a NanoDrop D-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE). A total of eleven paired-end libraries, including four types of small-insert libraries (180 bp, 260 bp, 300 bp, 500 bp) and seven large-insert libraries (2 Kb, 3 Kb, 4 Kb, 5 Kb, 6 Kb, 8 Kb, 20 Kb), were prepared following the Illumina’s instructions, and sequenced using Illumina HiSeq2000 platform by following the standard Illumina protocols (Illumina, San Diego, CA). |
WGA |
GENOMIC |
unspecified |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 151
Planned read length (bp) for mate 2: 151
Insert size (bp): 260
|
Release date | 2019-05-14 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR055191 |
CRR055191_f1.fastq.gz
CRR055191_r2.fastq.gz
|
8,897.78
9,071.81
|
CRR055192 |
CRR055192_f1.fastq.gz
CRR055192_r2.fastq.gz
|
7,425.77
7,613.55
|
CRR055193 |
CRR055193_f1.fastq.gz
CRR055193_r2.fastq.gz
|
11,616.89
11,819.16
|
|
Submitter | Fen Zhang (zhangfen@stu.scau.edu.cn) |
Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
Date submitted | 2019-05-06 |