Experiment information
Accession CRX049511
Organism Anser sp.
Title Differential gene expression profiling of the goose laying period pineal organ
BioProject PRJCA001411
BioSample SAMC068937
Platform Illumina HiSeq 2000
Library
Library name Construction protocol Strategy Source Selection Layout
laying period and broodiness period libraries Total RNA was extracted from each pineal organ using TRIzol reagent (Invitrogen, USA) according to the manufacturer’s instructions. RNA quality was confirmed on 1% agarose gels. RNA purity and concentration was determined using a NanoPhotometer® spectrophotometer (IMPLEN, CA, USA) and Qubit® RNA Assay Kit (Qubit® 2.0 Flourometer; Life Technologies, CA, USA) according to the manufacturer’s instructions. RNA integrity was assessed using the RNA 6000 Nano Assay Kit (Bioanalyzer 2100 system; Agilent Technologies, CA, USA). Sequencing libraries were generated using the NEBNext® Ultra™ RNA Library Prep Kit (Illumina®, NEB, USA) and following the manufacturer’s recommendations. Clustering of the index-coded samples was performed on a cBot Cluster Generation System using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina) according to the manufacturer’s instructions. After cluster generation, the library preparations were sequenced on an Illumina HiSeq platform and 125 bp/150 bp paired-end reads were generated. RNA-Seq GENOMIC PolyA SINGLE
Processing Planned read length (bp): 150
Release date2020-06-30
Run
Run accession Run data file information
File nameFile size (MB)
CRR055059 CRR055059.fq.gz 1,130.03
SubmitterXin Yuan (nihaoyuanxin88@outlook.com)
OrganizationInstitute of Animal Genetics and Breeding/SichuanAgricultural University
Date submitted2019-05-06
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