Experiment information
Accession CRX048750
Organism Arabidopsis thaliana
Title Root-scRNA-seq
BioProject PRJCA001393
BioSample SAMC068470
Platform Illumina NovaSeq 6000
Library
Library name Construction protocol Strategy Source Selection Layout
The root tip region (0.5 cm in length from root tip) were harvested and digested for 2 hours at room temperature in the RNase-free enzyme solution (1.5% cellulase R10, 1.5% macerozyme R10, 0.4 M mannitol, 10 mM KCl, 10 mM CaCl2 and 0.1% BSA). The protoplasts were filtered with a cell strainer (40 in μm diameter, FALCON, 352340), concentrated and washed 3-4 times with 8% mannitol at room temperature. The protoplast viability is determined by trypan blue staining. The ratio of viable cell should be more than 85%. The concentration of protoplasts was adjusted to 1500 ~ 2000 cells/μL. The cells were then processed with the 10xGenomicsZhang et al: Arabidopsis root atlas Single Cell Protocol (CG00052, RevC) RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Insert size (bp): 500
Release date2019-05-06
Run
Run accession Release date Run data file information
File nameFile size (MB)
CRR054647 2019-05-06 Root_R1.tar.gz
Root_R2.fastq.gz
7,850.21
24,424.1
SubmitterZhougeng Xu (xuzhougeng@163.com)
OrganizationsInstitute of Plant Physiology & Ecology, Chinese Academy of Sciences
Date submitted2019-04-26
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