Experiment information
Accession CRX059210
Organism Ovis aries
Title we sequence this Kazakh sheep KAZ64 with whole-genome resequencing
BioProject PRJCA001221
BioSample SAMC056153
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
We used no less than 18 µg (i.e., > 20 ng/µl and > 900 µl) of genomic DNA per sample to construct the libraries. DNA was sheared into fragments of 200 – 800 bp with the Covaris system (Covaris, Woburn, MA). The fragments were then treated following the Illumina DNA sample preparation protocol, with a process of end repaired, A-tailed, ligated to paired-end adaptors and PCR amplification with 350 bp inserts. The constructed libraries were sequenced on the Illumina HiSeq X Ten platform for 274 paired-end reads. WGS GENOMIC RANDOM PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
Release date2019-10-01
Run
Run accession Release date Run data file information
File nameFile size (MB)
CRR069658 2019-10-01 BFC2016518-01-HS64_H7NTHALXX_L6_1.fq.gz
BFC2016518-01-HS64_H7NTHALXX_L6_2.fq.gz
5,322.35
6,433.66
Submitter朱 强辉 (1337657902@qq.com)
OrganizationsInstitute of Zoology, Chinese Academy of Sciences
Date submitted2019-08-21
Related experiments
Experiments(185)