Accession | CRX048241 |
Organism | Ovis aries |
Title | we sequence this Tan sheep TAN18 with whole-genome resequencing |
BioProject | PRJCA001221 |
BioSample | SAMC056182 |
Platform | Illumina HiSeq X Ten |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
We used no less than 18 µg (i.e., > 20 ng/µl and > 900 µl) of
genomic DNA per sample to construct the libraries. DNA was sheared into fragments
of 200 – 800 bp with the Covaris system (Covaris, Woburn, MA). The fragments were
then treated following the Illumina DNA sample preparation protocol, with a process
of end repaired, A-tailed, ligated to paired-end adaptors and PCR amplification with
350 bp inserts. The constructed libraries were sequenced on the Illumina HiSeq X Ten
platform for 303 paired-end reads. |
WGS |
GENOMIC |
RANDOM PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
|
Release date | 2019-10-01 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR053318 |
CRR053318_f1.fastq.gz
CRR053318_r2.fastq.gz
|
5,663.76
6,239.08
|
|
Submitter | ? ?? (1337657902@qq.com) |
Organization | Institute of Zoology, Chinese Academy of Sciences |
Date submitted | 2019-04-18 |