Accession | CRX048224 |
Organism | Ovis aries |
Title | we sequence this Lanzhou Large-tailed sheep LAN6 with whole-genome resequencing |
BioProject | PRJCA001221 |
BioSample | SAMC056165 |
Platform | Illumina HiSeq X Ten |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
We used no less than 18 µg (i.e., > 20 ng/µl and > 900 µl) of
genomic DNA per sample to construct the libraries. DNA was sheared into fragments
of 200 – 800 bp with the Covaris system (Covaris, Woburn, MA). The fragments were
then treated following the Illumina DNA sample preparation protocol, with a process
of end repaired, A-tailed, ligated to paired-end adaptors and PCR amplification with
350 bp inserts. The constructed libraries were sequenced on the Illumina HiSeq X Ten
platform for 286 paired-end reads. |
WGS |
GENOMIC |
RANDOM PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
|
Release date | 2019-10-01 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR053295 |
CRR053295_f1.fq.gz
CRR053295_r2.fq.gz
|
3,667.52
4,422.55
|
CRR053296 |
CRR053296_f1.fq.gz
CRR053296_r2.fq.gz
|
3,352.53
4,075.67
|
|
Submitter | ? ?? (1337657902@qq.com) |
Organization | Institute of Zoology, Chinese Academy of Sciences |
Date submitted | 2019-04-18 |