Experiment information
Accession CRX048208
Organism Ovis aries
Title we sequence this Hulun Buir sheep HLS23 with whole-genome resequencing
BioProject PRJCA001221
BioSample SAMC056149
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
We used no less than 18 µg (i.e., > 20 ng/µl and > 900 µl) of genomic DNA per sample to construct the libraries. DNA was sheared into fragments of 200 – 800 bp with the Covaris system (Covaris, Woburn, MA). The fragments were then treated following the Illumina DNA sample preparation protocol, with a process of end repaired, A-tailed, ligated to paired-end adaptors and PCR amplification with 350 bp inserts. The constructed libraries were sequenced on the Illumina HiSeq X Ten platform for 270 paired-end reads. WGS GENOMIC RANDOM PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
Release date2019-10-01
Run
Run accession Run data file information
File nameFile size (MB)
CRR053273 CRR053273_f1.fq.gz
CRR053273_r2.fq.gz
3,451.62
3,877.54
CRR053274 CRR053274_f1.fq.gz
CRR053274_r2.fq.gz
3,145.06
3,553.34
Submitter? ?? (1337657902@qq.com)
OrganizationInstitute of Zoology, Chinese Academy of Sciences
Date submitted2019-04-18
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