| Accession | CRX048183 |
| Organism | Ovis aries |
| Title | we sequence this Tengchong sheep TCS5 with whole-genome resequencing |
| BioProject | PRJCA001221 |
| BioSample | SAMC056124 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
We used no less than 18 µg (i.e., > 20 ng/µl and > 900 µl) of
genomic DNA per sample to construct the libraries. DNA was sheared into fragments
of 200 – 800 bp with the Covaris system (Covaris, Woburn, MA). The fragments were
then treated following the Illumina DNA sample preparation protocol, with a process
of end repaired, A-tailed, ligated to paired-end adaptors and PCR amplification with
350 bp inserts. The constructed libraries were sequenced on the Illumina HiSeq X Ten
platform for 245 paired-end reads. |
WGS |
GENOMIC |
RANDOM PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
|
| Release date | 2019-10-01 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR053236 |
CRR053236_f1.fq.gz
CRR053236_r2.fq.gz
|
4,514.23
5,367.52
|
|
| Submitter | ? ?? (1337657902@qq.com) |
|---|
| Organization | Institute of Zoology, Chinese Academy of Sciences |
|---|
| Date submitted | 2019-04-18 |
|---|
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