Experiment information
Accession CRX048029
Organism Arabidopsis thaliana
Title pif4-2-22-2 Arabidopsis
BioProject PRJCA001375
BioSample SAMC067802
Platform Illumina HiSeq 2500
Library name Construction protocol Strategy Source Selection Layout
Approximately 3 µg of total RNA was used for isolating poly(A) mRNA with Poly(A) mRNA Magnetic Isolation Module (NEB). Following purification, the mRNA was fragmented into small pieces by adding fragmentation buffer. Then, the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the NEBNext Ultua RNA Library Prep Kit (NEB). The average insert size for the paired-end libraries was 300 bp. Then, paired-end sequencing (100 bp) was carried out on the HiSeq2500 platform (Illumina). RNA-Seq TRANSCRIPTOMIC PolyA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
Release date2019-07-07
Run accession Release date Run data file information
File nameFile size (MB)
CRR053081 2019-07-07 pif4-2-22-2_combined_R1.fastq.gz
Submitterhuanhuan Jin (1152225800@qq.com)
OrganizationsNanjing Normal University
Date submitted2019-04-15
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