Experiment information
Accession CRX044289
Organism Fusarium oxysporum f. sp. cubense
Title Whole-genome sequencing of Foc1 60
BioProject PRJCA001282
BioSample SAMC063841
Platform PacBio Sequel
Library name Construction protocol Strategy Source Selection Layout
Foc1 60 genome sequencing DNA was extracted using the cetyltrimethylammonium bromide (CTAB) method, 20-kb inserts were constructed using the SMRTbellTM Template Prep Kit 1.0.Then was mechanically sheared by Covaris g-TUBE (Covaris, Woburn, MA, USA), followed by DNA damage repair and end-repair before ligating the hairpin adaptors to generate the SMRTbell template. A Blue Pippin device (Safe Science Inc., Beverly, MA, USA) was used for size selection with a cut-off value of 9,000 bp, and the size-selected library was purified using AMPure PB beads (Beckman Coulter, High Wycombe, UK).Then it was sequenced on PacBio Sequel system. WGS GENOMIC unspecified FRAGMENT
Release date2019-04-28
Run accession Release date Run data file information
File nameFile size (MB)
CRR049237 2019-04-28 Foc1_60.subreads.tar.gz 5,617.85
SubmitterLiangsheng Zhang (fafuzhang@163.com)
OrganizationsBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2019-03-10
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