Accession | CRX042096 |
Organism | Camellia sinensis |
Title | GBS:Tea 397 |
BioProject | PRJCA001272 |
BioSample | SAMC059090 |
Platform | Illumina HiSeq X Ten |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total genomic DNA of each tea plant sample was extracted from fresh leaves using plant genomic DNA rapid extraction kit (Biomed Gene Technology). The integrity, purity, and concentration of the extracted DNA were determined by 1% agarose gel electrophoresis and a Qubit Fluorometer (Invitrogen). One-hundred nanogram genomic DNA was digested with restriction enzymes SacI and MseI. The resulting samples were ligated with the restriction fragment using the designed sequence linker. The PCR ampli?cations were used to enrich the adapter-ligated DNA fragments. The inserted fragments with a length of 370-420bp were selected. Finally, the selected DNA fragments were enriched, amplified and sequenced using the Illumina HiSeq X ten platform basing on the Paired-End 140 (PE140) strategy. |
OTHER |
GENOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 140
Planned read length (bp) for mate 2: 140
Insert size (bp): 395
|
Release date | 2019-07-31 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR046850 |
CRR046850_f1.fq.gz
CRR046850_r2.fq.gz
|
197.11
227.91
|
|
Submitter | SuZhen Niu (niusuzhen@163.com) |
Organization | College of Tea Science, Guizhou University |
Date submitted | 2019-02-22 |