Experiment information
Accession CRX041688
Organism Pyrus communis
Title P. communis 'Abate Fetel'
BioProject PRJCA001284
BioSample SAMC058636
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
High-quality genomic DNA was randomly interrupted by ultrasound. DNA fragments ranging from 150~800 bp were recovered by electrophoresis. T4 DNA Polymerase, Klenow DNA Polymerase and T4 PNK were used to repair cohesive ends to blunt ends. The 30 end of DNA fragment was amended including an “A” base and ligated to adaptors, including a 50 end T base. All fragments were recovered by electrophoresis and then paired-end sequenced by an Illumina HiSeq 4000. WGA GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 500
Release date2019-03-14
Run
Run accession Run data file information
File nameFile size (MB)
CRR046442 CRR046442_f1.fastq.gz
CRR046442_r2.fastq.gz
2,217.2
2,415.15
SubmitterShuang Jiang (271339616@qq.com)
OrganizationShanghai Academy of Agricultural Sciences
Date submitted2019-02-19
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Experiments(4)