Accession | CRX074845 |
Organism | Mus musculus |
Title | Early-2-cell_rep4 |
BioProject | PRJCA001255 |
BioSample | SAMC116608 |
Platform | Illumina HiSeq X Ten |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The generation of Hi-C libraries with a low number of cells was optimized according to a previous protocol. For Hi-C library generation and sequencing, 100-500 cells were used per reaction, at least two replicates were performed for each stage. All isolated cells were washed three times in 0.5% bovine serum albumin in phosphate-buffered saline (BSA-PBS, Sigma) solution to avoid potential contamination. |
Hi-C |
GENOMIC |
unspecified |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
|
Release date | 2020-04-15 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR087774 |
CRR087774_f1.fq.gz
CRR087774_r2.fq.gz
|
39,062.22
40,751.05
|
|
Submitter | Qianshu Zhu (zhuqianshu@tongji.edu.cn) |
Organization | Tongji University |
Date submitted | 2019-11-27 |