Experiment information
Accession CRX045525
Organism Homo sapiens
Title Huh7_SP2_HiC
BioProject PRJCA001245
BioSample SAMC065117
Platform Illumina NovaSeq 5000
Library name Construction protocol Strategy Source Selection Layout
The Hi-C experiment was performed follow the in-situ Hi-C protocol. Briefly, cells were lysis and chromatin was digested by MobI, then biotin fill-in and Hi-C ligation. After ligation, DNA was purified and sonicated to 200-500bp for library construction follow the . DNA was end-repaired, dATP attached and adapter ligated follow the in-situ Hi-C protocl. After adapter ligation DNA was PCR amplified and library fragments between 200-500bp were purified for sequencing. The purified library was sequenced on NovaSeq at BerryGenomics Co., LTD. Hi-C GENOMIC Restriction Digest PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
Release date2019-10-01
Run accession Release date Run data file information
File nameFile size (MB)
CRR050458 2019-10-01 SP2-HiC_R1.clean.fq.gz
SubmitterXiaoyuan Song (songxy5@ustc.edu.cn)
OrganizationsUniversity of Science and Technology of China
Date submitted2019-03-22