Experiment information
Accession CRX040237
Organism Homo sapiens
Title A survey of the transcriptional landscape in esophageal squamous cells with long read single molecule real-time sequencing
BioProject PRJCA001228
BioSample SAMC055206
Platform PacBio Sequel
Library
Library name Construction protocol Strategy Source Selection Layout
The libraries was prepared according to the Isoform Sequencing protocol (Iso-Seq) as described by Pacific Biosciences (PN100-092-800-03). Firstly the cDNA was synthesized by total mRNAs for each sample using the Clontech SMARTer PCR cDNA Synthesis Kit. To increase the sequencing yield of long transcripts, the Blue Pippin Size Selection System protocol was used to select the >4kb fragments for each sample after the PCR amplification of the cDNAs. Then the equi-molar mixture of long cDNA fragments (>4kb) and normal cDNAs were used subsequently for SMRT sequencing. Synthetic-long-Read TRANSCRIPTOMIC PCR SINGLE
Processing
Release date2019-07-10
Run
Run accession Run data file information
File nameFile size (MB)
CRR044980 CRR044980.tar.gz 3,202.89
CRR044981 CRR044981.tar.gz 6,625.16
CRR044982 CRR044982.tar.gz 6,514.55
SubmitterYunmei Chen (chenyunmei@novogene.com)
OrganizationNovogene
Date submitted2019-01-19