Experiment information
Accession CRX038450
Organism Paeonia suffruticosa
Title stamen transcriptome
BioProject PRJCA000701
BioSample SAMC053486
Platform BGISEQ-500
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted using CTAB-LiCl method,and genomic DNA contamination was removed using DNase I (TaKaRa, Dalian, China).RNA quality was verified by agarose gel electrophoresis and a Bioanalyzer 2100 (Agilent,CA, USA). For cDNA library construction, mRNA was enriched with oligo (dT) magnetic beads and then broken into smaller pieces using fragmentation buffer. The first strand cDNA was reversed-transcribed by random hexamers and small fragment as templates.This was followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. The double strand cDNA was ligated to paired-end adapters, and suitable fragments were selected and enriched with PCR amplification. RNA-Seq TRANSCRIPTOMIC unspecified PAIRED
Processing Insert size (bp): 101
Release date2019-12-31
Run
Run accession Run data file information
File nameFile size (MB)
CRR043123 CRR043123_f1.fq.gz
CRR043123_r2.fq.gz
2,542.16
2,838.32
SubmitterZhang Yanzhao (landscape031@163.com)
OrganizationLuoyang Normal University
Date submitted2019-01-10
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