Experiment information
Accession CRX034194
Organism Homo sapiens
Title Colorectal adenocarcinoma cell line MeRIP-Seq
BioProject PRJCA001142
BioSample SAMC049550
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
50 μg of total RNA was extracted and purified using RiboMinus™ Eukaryote Kit v2 (Invitrogen) to deplete the ribosomal RNA from the total RNA. RNA Fragmentation Reagents were used to shear the RNA into approximately 100 nt in length. 1/10 of the fragmented RNA were saved as the input control. The rest of the RNA were incubated with an anti-m6A antibody (202203, Synaptic Systems) for one hour 4°C, and was then mixed with prewashed Protein A/G magnetic beads in immunoprecipitation buffer at 4°C overnight. The m6A antibody was digested with proteinase K digestion buffer and the methylated RNA was purified for further MeRIP sequencing. RIP-Seq TRANSCRIPTOMIC other SINGLE
Processing Planned read length (bp): 50
Release date2019-10-29
Run
Run accession Run data file information
File nameFile size (MB)
CRR038727 CRR038727.fq.gz 1,330.15
SubmitterXingyang Li (yang9224822@gzmu.net.cn)
OrganizationSun Yat-sen University
Date submitted2018-11-26