| Accession | CRX034185 |
| Organism | Arabidopsis thaliana |
| Title | Poly(A)_Col |
| BioProject | PRJCA001119 |
| BioSample | SAMC049541 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was extracted from the two-week-old seedlings of Col. 5 ?g of total RNA was treated with RQ1 DNase (Promega, M6101) and was partially digested by RNase T1 (Thermo, EN0541), an endoribonuclease that specifically digests single-stranded RNA at the 3?-side of the G residue. Poly(A)-containing RNA fragments were purified with oligo(dT)-conjugated magnetic beads (Invitrogen, 61005) and was ligated to the 3?-RNA adaptor. The cDNA was synthesized using ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Illumina, SSV21124). PCR products of 200-500 bp were purified and were applied to HiSeq X ten system for 150-nt pair-end sequencing. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
| Release date | 2020-11-16 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR038718 |
CRR038718_f1.fq.gz
CRR038718_r2.fq.gz
|
2,439.28
2,185.28
|
|
| Submitter | Qing Huan (qhuan@genetics.ac.cn) |
|---|
| Organization | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences |
|---|
| Date submitted | 2018-11-26 |
|---|
English
中文
Français