| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
0.1 g two-week-old seedlings were transferred to 2 ml incubation buffer (1mM PIPES, pH 6.25, 1 mM Sodium Citrate, 1 mM KCl, 15 mM Sucrose) in a 24-well plate. Cordycepin was added to a final concentration of 0.5 mM. The plate was rotated for 75 rpm at 23°C. The seedlings were harvested at the time point 0, 0.5, 1, 2, 4, and 8 hours and were quickly frozen in liquid nitrogen. Total RNA was isolated with TRIzol (Invitrogen, 15596026). mRNA-seq libraries were constructed using the strand-specific protocol for Illumina sequencing and were sequenced with HiSeq X ten system in the pair-end 150-nt mode. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
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