Accession | CRX034171 |
Organism | Arabidopsis thaliana |
Title | Degradation_Col_0.5h_rep2 |
BioProject | PRJCA001119 |
BioSample | SAMC049527 |
Platform | Illumina HiSeq X Ten |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
0.1 g two-week-old seedlings were transferred to 2 ml incubation buffer (1mM PIPES, pH 6.25, 1 mM Sodium Citrate, 1 mM KCl, 15 mM Sucrose) in a 24-well plate. Cordycepin was added to a final concentration of 0.5 mM. The plate was rotated for 75 rpm at 23°C. The seedlings were harvested at the time point 0, 0.5, 1, 2, 4, and 8 hours and were quickly frozen in liquid nitrogen. Total RNA was isolated with TRIzol (Invitrogen, 15596026). mRNA-seq libraries were constructed using the strand-specific protocol for Illumina sequencing and were sequenced with HiSeq X ten system in the pair-end 150-nt mode. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
Release date | 2020-11-16 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR038704 |
CRR038704_f1.fastq.gz
CRR038704_r2.fastq.gz
|
1,404.34
1,557.41
|
|
Submitter | Qing Huan (qhuan@genetics.ac.cn) |
Organization | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences |
Date submitted | 2018-11-26 |