| HepG2 total RNAseq library |
Total RNA were firstly extracted from HepG2 cells using the TransZol Up kit (#ET111, TransGen), the ribosomal RNAs (rRNAs) in the total RNA samples were depleted using the Epicentre Ribo-Zero rRNA Removal Kit (illumina, USA). Eventually, 1μg rRNA-depleted total RNAs were used for library preparation. The RNAs were fragmented into about 300bp for cDNA generation. The sequencing was performed on HiSeq 3000/4000 PE Cluster Kit with the read length equal to 150bp. |
RNA-Seq |
TRANSCRIPTOMIC |
unspecified |
PAIRED
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