Experiment information
Accession CRX032469
Organism Saccharomyces cerevisiae
Title treatment_1 h_1
BioProject PRJCA001109
BioSample SAMC048005
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
The total RNA was extracted from the collected sample by using a Spin Column Yeast Total RNA Purification Kit (Sangon Biotech, China). A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v4-cBot-HS (Illumia) according to the manufacturer’s instructions. After cluster generation, the library preparations were sequenced on an Illumina platform and paired-end reads were generated. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 280
Release date2020-11-13
Run
Run accession Run data file information
File nameFile size (MB)
CRR036636 CRR036636_f1.fq.gz
CRR036636_r2.fq.gz
1,466.9
1,664.03
SubmitterXiaopeng Guo (guoxp@impcas.ac.cn)
OrganizationInstitute of Modern Physics, Chinese Academy of Sciences
Date submitted2018-11-15