Experiment information
Accession CRX032436
Organism Strongylocentrotus purpuratus
Title sea urchin sperm methylome rep1
BioProject PRJCA000098
BioSample SAMC047984
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Genomic DNA spiked with 0.5? unmethylated Lambda DNA (Promega) was sonicated into 100-500bp. Fragmented DNA was then subjected to end repair,dA tailing, ligation with the end repair enzyme mix (NEB), Klenow 3´-5´ exo- (NEB) and T4 DNA ligase (NEB), respectively. Adapter-ligated DNA was recovered by 2? agarose gel electrophoresis, and bisulfite conversion was performed using the EZ DNA methylation-Gold kit (Zymo Research) according to the instruction manual. Bisulfite-treated DNA was amplified with KAPA HiFi HotStart Uracil+ ReadyMix. Amplified DNA was recovered by 2? agarose gel electrophoresis. Bisulfite-Seq GENOMIC RANDOM PAIRED
Processing Planned read length (bp) for mate 1: 125
Planned read length (bp) for mate 2: 125
Insert size (bp): 300
Release date2019-05-30
Run
Run accession Run data file information
File nameFile size (MB)
CRR036572 CRR036572_f1.fastq.gz
CRR036572_r2.fastq.gz
4,286.12
3,964.52
CRR036573 CRR036573_f1.fastq.gz
CRR036573_r2.fastq.gz
9,324.71
8,851.97
CRR036574 CRR036574_f1.fastq.gz
CRR036574_r2.fastq.gz
10,075.74
9,603.17
Submitterxiaocui xu (xuxc@big.ac.cn)
OrganizationBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2018-11-13