Experiment information
Accession CRX032348
Organism Mus musculus
Title Lnc20-1
BioProject PRJCA001103
BioSample SAMC047891
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
Germ cells were isolated from the AAV9-injected testes and made into single-cell suspensions. RFP positive germ cells were isolated by FACS.3000 cells in each sample, including 3000 shCtrl, 3000 lnc20-shRNA1# and 3000 lnc20-shRNA2# germ cells were prepared for library construction. Single-cell RNA-seq libraries were prepared using Single Cell 3' Library Gel Bead Kit V2 (10x Genomics) following the manufacture’s introduction. RNA-Seq TRANSCRIPTOMIC cDNA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 423
Release date2021-01-12
Run
Run accession Run data file information
File nameFile size (MB)
CRR036241 CRR036241_f1.fastq.gz
CRR036241_r2.fastq.gz
52,986.76
45,751.99
SubmitterXu Jiayue (xujiayue78@126.com)
OrganizationPeking Union Medical College
Date submitted2018-11-06