Accession | CRX032348 |
Organism | Mus musculus |
Title | Lnc20-1 |
BioProject | PRJCA001103 |
BioSample | SAMC047891 |
Platform | Illumina HiSeq X Ten |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Germ cells were isolated from the AAV9-injected testes and made into single-cell suspensions. RFP positive germ cells were isolated by FACS.3000 cells in each sample, including 3000 shCtrl, 3000 lnc20-shRNA1# and 3000 lnc20-shRNA2# germ cells were prepared for library construction. Single-cell RNA-seq libraries were prepared using Single Cell 3' Library Gel Bead Kit V2 (10x Genomics) following the manufacture’s introduction. |
RNA-Seq |
TRANSCRIPTOMIC |
cDNA |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 423
|
Release date | 2021-01-12 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR036241 |
CRR036241_f1.fastq.gz
CRR036241_r2.fastq.gz
|
52,986.76
45,751.99
|
|
Submitter | Xu Jiayue (xujiayue78@126.com) |
Organization | Peking Union Medical College |
Date submitted | 2018-11-06 |