Experiment information
Accession CRX031453
Organism Rattus norvegicus
Title Kcnip3 KO_3
BioProject PRJCA001068
BioSample SAMC047342
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
RNA isolation was performed as described above. Genomic DNA was removed by gDNA removal column provided in the kit. An Agilent RNA Nano Kit and an Agilent 2100 Bioanalyzer (Santa Clara, CA, USA) were used for RNA integrity and concentration detection. For each sample, 5 ?g of total RNA was used to construct the Illumina sequencing libraries according to the manufacturer’s instructions. The libraries were sequenced using the Illumina HiSeq X Ten platform to generate high-quality paired-end reads of 150 nt. Rattus norvegicus genome sequences and annotated gene models were downloaded from ENSEMBL (Rnor6). Raw sequencing reads were first processed to remove adaptors and low-quality bases using Fastqc and Trimmomatic and then aligned to reference genome sequences using STAR (2.5.2b) with gene annotation indexed. The mapping quality and saturation analysis were performed using RSeQC. RNA-Seq TRANSCRIPTOMIC PolyA PAIRED
Processing Planned read length (bp): 150
Release date2020-10-13
Run
Run accession Run data file information
File nameFile size (MB)
CRR035322 CRR035322_f1.fq.gz
CRR035322_r2.fq.gz
1,228.1
1,314.51
SubmitterYing Zhang (zhangyingnri@bjmu.edu.cn)
OrganizationPeking University
Date submitted2018-10-19
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