Accession | CRX031453 |
Organism | Rattus norvegicus |
Title | Kcnip3 KO_3 |
BioProject | PRJCA001068 |
BioSample | SAMC047342 |
Platform | Illumina HiSeq 4000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
RNA isolation was performed as described above. Genomic DNA was removed by gDNA removal column provided in the kit. An Agilent RNA Nano Kit and an Agilent 2100 Bioanalyzer (Santa Clara, CA, USA) were used for RNA integrity and concentration detection. For each sample, 5 ?g of total RNA was used to construct the Illumina sequencing libraries according to the manufacturer’s instructions. The libraries were sequenced using the Illumina HiSeq X Ten platform to generate high-quality paired-end reads of 150 nt.
Rattus norvegicus genome sequences and annotated gene models were downloaded from ENSEMBL (Rnor6). Raw sequencing reads were first processed to remove adaptors and low-quality bases using Fastqc and Trimmomatic and then aligned to reference genome sequences using STAR (2.5.2b) with gene annotation indexed. The mapping quality and saturation analysis were performed using RSeQC. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
Processing |
Planned read length (bp): 150
|
Release date | 2020-10-13 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR035322 |
CRR035322_f1.fq.gz
CRR035322_r2.fq.gz
|
1,228.1
1,314.51
|
|
Submitter | Ying Zhang (zhangyingnri@bjmu.edu.cn) |
Organization | Peking University |
Date submitted | 2018-10-19 |