Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
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Total RNA was isolated from grape leaves by use of RNAprep Pure Plant Kit (Polysaccharides & Polyphenolic-rich, Tiangen, Beijing, China), and quantified with the Nanodrop system (Thermo Fisher Scientific). DNase digestion was performed during total RNA isolation to remove genomic DNA. A total amount of 3 ?g RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext UltraTM Directional RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and then fragmented and reverse transcribed to cDNA. Standard RNA-seq library was constructed based on Illumina protocol and subjected to sequencing using Illumina Hiseq X Ten, and paired-end reads were generated. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
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