Experiment information
Accession CRX030274
Organism Homo sapiens
Title HCC_dual_small_31
BioProject PRJCA001042
BioSample SAMC046619
Platform Illumina HiSeq 2500
Library name Construction protocol Strategy Source Selection Layout
Resected tumor sample was collected from a 46-year-old male patient and transported in DMEM (GIBCO) on ice immediately after surgical. Tumor pieces were digested with accumax to obtain single cell suspension. Single cells were picked up and lysed with 200 ng of the carrier RNA mixture (containing 100 ng 5’-phosphorylated small carrier RNA mimic). The poly-A RNAs were selected using a NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490), and the small RNAs remained in the supernatant after poly-A selection. The small RNA sequencing library was constructed following the manufacturer’s protocol using a NEBNext kit (NEB #E7300). After PCR amplification (15 cycles), the cDNA fragment from the RNA carrier was removed by Not I digestion. After Not I digestion, the library DNA was purified for deep sequencing. ncRNA-Seq TRANSCRIPTOMIC other FRAGMENT
Processing Planned read length (bp): 50
Release date2018-09-29
Run accession Release date Run data file information
File nameFile size (MB)
CRR033715 2018-09-29 HCC_dual_small_31.fastq.gz 324.33
SubmitterZhengyun Xiao (xiaozhengyun1993@126.com)
OrganizationsZhejiang University
Date submitted2018-09-23