Accession | CRX030210 |
Organism | Homo sapiens |
Title | BreastCancerSC_beforeCapt |
BioProject | PRJCA001042 |
BioSample | SAMC046555 |
Platform | Illumina HiSeq 2500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
A single cell was lysed with 100 ng of the carrier RNA mixture. The sequencing library was constructed following the manufacturer’s protocol using a NEBNext kit (E7420). After adaptor ligation, the cDNA fragments from the RNA carrier were removed by Not I digestion. After Not I digestion, the library DNA was purified for USER digestion and PCR amplification.PCR amplification was performed using primers containing 8-nt degenerate barcodes to perform the first 10 cycles of library amplification, followed by another 15 cycles of amplification by regular P5/P7 primers. |
RNA-Seq |
TRANSCRIPTOMIC |
RANDOM |
SINGLE
|
|
Processing |
Planned read length (bp): 50
|
Release date | 2018-09-29 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR033651 |
CRR033651.fastq.gz
|
324.59
|
|
Submitter | Zhengyun Xiao (xiaozhengyun1993@126.com) |
Organization | Zhejiang University |
Date submitted | 2018-09-23 |