Experiment information
Accession CRX030208
Organism Homo sapiens
Title 293T_total_sc5
BioProject PRJCA001042
BioSample SAMC046553
Platform Illumina HiSeq 2500
Library name Construction protocol Strategy Source Selection Layout
A single cell was lysed with 100 ng of the carrier RNA mixture. The sequencing library was constructed following the manufacturer’s protocol using a NEBNext kit (E7530). After adaptor ligation and USER digestion, cDNA fragments from the RNA carrier were removed by Not I digestion. After Not I digestion, the library DNA was purified for PCR amplification. RNA-Seq TRANSCRIPTOMIC RANDOM FRAGMENT
Processing Planned read length (bp): 50
Release date2018-09-29
Run accession Release date Run data file information
File nameFile size (MB)
CRR033649 2018-09-29 293T_total_sc5.fastq.gz 331.58
SubmitterZhengyun Xiao (xiaozhengyun1993@126.com)
OrganizationsZhejiang University
Date submitted2018-09-23