Experiment information
Accession CRX029235
Organism Anser indicus
Title Cloacae_2
BioProject PRJCA001005
BioSample SAMC045562
Platform Illumina MiSeq
Library name Construction protocol Strategy Source Selection Layout
Microbial DNA was extracted using the E.Z.N.A. stool DNA Kit (Omega Bio-tek, Norcross, GA, USA) according to the manufacturers protocols. The V4 - V5 hyper-variable region of the 16S rRNA genes was amplified with the universal primers 515F 5-(GTGCCAGCMGCCGCGG)-3 and 907R 5-(CCGTCAATTCMTTTRAGTTT)-3 by thermocycler PCR system (GeneAmp 9700, ABI, USA). Purified amplicons were pooled in equimolar and paired-end sequenced (2 X 300 bp) on an Illumina Miseq platform (Illumina, San Diego, USA) according to the standard protocols by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China). CLONE GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 300
Planned read length (bp) for mate 2: 300
Insert size (bp): 300
Release date2018-09-05
Run accession Release date Run data file information
File nameFile size (MB)
CRR032577 2018-09-05 raw.split.2_7.1.gz
SubmitterWen Wang (007cell@163.com)
OrganizationsQinghai University
Date submitted2018-09-04
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