Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
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Microbial DNA was extracted using the E.Z.N.A. stool DNA Kit (Omega Bio-tek, Norcross, GA, USA) according to the manufacturers protocols. The V4 - V5 hyper-variable region of the 16S rRNA genes was amplified with the universal primers 515F 5-(GTGCCAGCMGCCGCGG)-3 and 907R 5-(CCGTCAATTCMTTTRAGTTT)-3 by thermocycler PCR system (GeneAmp 9700, ABI, USA). Purified amplicons were pooled in equimolar and paired-end sequenced (2 X 300 bp) on an Illumina Miseq platform (Illumina, San Diego, USA) according to the standard protocols by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China). |
CLONE |
GENOMIC |
PCR |
PAIRED
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