Experiment information
Accession CRX030745
Organism Danio rerio
Title MOpabpc1a_6hpf_rep2_RNA-seq
BioProject PRJCA001013
BioSample SAMC047056
Platform Illumina HiSeq 2500
Library name Construction protocol Strategy Source Selection Layout
Total RNA was isolated from zebrafish embryos harvested at different stages using TRIzol® Reagent (Ambion). mRNA was extracted with using Dynabeads® mRNA Purification Kit (Ambion) and subjected to TURBO™ DNase (Invitrogen) treatment at 37°C for 30 min and ethanol precipitation. Thus purified mRNA was used for RNA-Seq library construction. Libraries were directly constructed using the KAPA Stranded mRNA-Seq Kit (KAPA) and were sequenced using HiSeq2500 (Illumina) in paired-read mode, creating reads with a length of 125 bp. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 125
Planned read length (bp) for mate 2: 125
Insert size (bp): 240
Release date2019-06-12
Run accession Release date Run data file information
File nameFile size (MB)
CRR034489 2019-06-12 MOpabpc1a_6hpf_rep2_R1.fastq.gz
Submittersun baofa (sunbf@big.ac.cn)
OrganizationsBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2018-10-12