| Accession | CRX029178 |
| Organism | Danio rerio |
| Title | iCLIP_4hpf_rep2_Ybx1-iCLIP |
| BioProject | PRJCA001013 |
| BioSample | SAMC045548 |
| Platform | Illumina HiSeq 2500 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
1000 zebrafish embryos at 4 hpf were irradiated twice with 0.8 J/cm2 (Stratalinker 2400, Stratagene), lysed and subjected to mild fragmentation. Crosslinked RNA-protein complexes were immunoprecipated using polyclonal Ybx1 antibody (Abmax) and protein A dynabeads. RNA was extracted and subjected to library construction using Smarter smRNA-Seq kit (Takara). All the libraries were sequenced using HiSeq2500 (Illumina) in paired-read mode, creating reads with a length of 150 bp. |
Targeted-Capture |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 250
|
| Release date | 2019-06-12 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR032497 |
CRR032497_f1.fastq.gz
CRR032497_r2.fastq.gz
|
2,802.36
4,592.75
|
|
| Submitter | sun baofa (sunbf@big.ac.cn) |
|---|
| Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
|---|
| Date submitted | 2018-08-31 |
|---|
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