Accession | CRX029159 |
Organism | Danio rerio |
Title | WT_0hpf_rep1_RNA-seq |
BioProject | PRJCA001013 |
BioSample | SAMC045529 |
Platform | Illumina HiSeq 2500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was isolated from zebrafish embryos harvested at different stages using TRIzol® Reagent (Ambion). mRNA was extracted with using Dynabeads® mRNA Purification Kit (Ambion) and subjected to TURBO™ DNase (Invitrogen) treatment at 37°C for 30 min and ethanol precipitation. Thus purified mRNA was used for RNA-Seq library construction. Libraries were directly constructed using the KAPA Stranded mRNA-Seq Kit (KAPA) and were sequenced using HiSeq2500 (Illumina) in paired-read mode, creating reads with a length of 125 bp. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 125
Planned read length (bp) for mate 2: 125
Insert size (bp): 240
|
Release date | 2019-06-12 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR032478 |
CRR032478_f1.fastq.gz
CRR032478_r2.fastq.gz
|
4,810.48
5,138.23
|
|
Submitter | sun baofa (sunbf@big.ac.cn) |
Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
Date submitted | 2018-08-31 |