Experiment information
Accession CRX029082
Organism Wohlfahrtia magnifica
Title Wohlfahrtia magnifica larvaLW1-3
BioProject PRJCA001000
BioSample SAMC045129
Platform Illumina HiSeq 4000
Library name Construction protocol Strategy Source Selection Layout
Wohlfahrtia magnifica larvae transcriptome database After the total RNA extracted from the sample passed the quality test, the eukaryotic mRNA was enriched using magnetic beads attached to Oligo (dT). The extracted mRNA was randomly broken into short fragments by Fragmentation Buffer, and the fragmented mRNA was used as a template to synthesize a strand cDNA with six-base random primers (Random hexamers), followed by buffer, dNTPs, RNaseH and DNA Polymerase I were subjected to double-stranded cDNA synthesis. AMPure XP beads purified double-stranded product, using T4 DNA polymerase and Klenow DNA polymerase activity to repair the sticky end of DNA to blunt end, 3' end plus base A and linker, AMPureXP beads for fragment selection, then USER enzyme The second strand of the cDNA containing U was degraded for PCR amplification to obtain a final sequencing library. After the library was qualified, it was sequenced using Illumina Hiseq4000, and the sequencing read length was double-ended 2*150 bp (PE150). RNA-Seq OTHER PolyA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 100
Release date2019-09-18
Run accession Release date Run data file information
File nameFile size (MB)
CRR032402 2019-09-18 LW1_3_R1.fastq.gz
Submitterwang chao (sy419419419@163.com)
OrganizationsInner Mongolia Agricultural University
Date submitted2018-08-28