Accession | CRX028913 |
Organism | Astragalus adsurgens |
Title | Erect milkvetch 2 |
BioProject | PRJCA000988 |
BioSample | SAMC044898 |
Platform | Illumina HiSeq 2000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
Erect milkvetch library |
The poly (A) mRNA was isolated by magnetic oligo (dT) beads, fragmentation buffer was added at appropriate temperatures to breaking the mRNA into short fragments. First-strand cDNA was synthesized by the interrupted mRNA as a template using random hexamer-primer. The second-strand cDNA was synthesized using buffer, dNTPs, RNaseH and DNA polymerase I. Short fragments were purified using Min Elute PCR Purification Kit (Qiagen, China), sticky-end repair and tailing A. Then, the short fragments were ligated to sequencing adapters, selected the suitable size of the fragment by the 1% agarose gel electrophoresis and performed the PCR amplification. Finally, the library was sequenced using Illumina HiSeqTM 2000 platform |
RNA-Seq |
TRANSCRIPTOMIC |
DNAse |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 150
|
Release date | 2019-08-17 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR032203 |
CRR032203_f1.fq.gz
CRR032203_r2.fq.gz
|
2,022.7
2,317.74
|
CRR032204 |
CRR032204_f1.fq.gz
CRR032204_r2.fq.gz
|
2,428.21
2,581.35
|
CRR032205 |
CRR032205_f1.fq.gz
CRR032205_r2.fq.gz
|
2,612.59
3,081.13
|
|
Submitter | zan wang (wangzan@caas.cn) |
Organization | Institute of Animal Science, Chinese Academy of Agricultural Sciences |
Date submitted | 2018-08-17 |