Experiment information
Accession CRX028839
Organism Homo sapiens
Title siNSUN2-T_rep2
BioProject PRJCA000975
BioSample SAMC044837
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was isolated using TRIzol® Reagent (Ambion). The RNA concentration and quality were determined with NanoDrop, Qubit and agarose gel analysis. The Dynabeads® mRNA Purification Kit (Ambion) was used to enrich the mRNAs, which were used as templates for RNA-Seq library construction. RNA-Seq libraries was directly constructed by using KAPA mRNA Stranded mRNA-Seq Kit (KAPA) according to the manufacturer’s instructions. The libraries were sequenced on the Illumina HiSeq X-Ten platform at Novogene (Tianjin, CA) with paired-end 150 bp read length. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
Release date2019-06-11
Run
Run accession Run data file information
File nameFile size (MB)
CRR032080 CRR032080_f1.fastq.gz
CRR032080_r2.fastq.gz
3,847.93
4,473.55
Submittersun baofa (sunbf@big.ac.cn)
OrganizationBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2018-08-10