Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
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FFPE tissue was gravity collected into collection tubes. Paraffin is removed from FFPE LCM sections using Deparaffinization Solution (Qiagen) and DNA extraction is performed using AllPrep DNA/RNA FFPE kit (Qiagen) according to AllPrep DNA/RNA FFPE Handbook. Isolated genomic DNA concentration was verified using the Qubit dsDNA HS Assay Kit (Life Technologies, Carlsbad, California, USA). Samples were prepared as described in the Ion AmpliSeq Library Preparation User Guide, Publication #MAN0006735 Rev. 5.0 (Life Technologies) using the Ion AmpliSeq Library Kit 2.0 with Ion AmpliSeq Comprehensive Cancer Panel v2.0 (Life Technologies). |
AMPLICON |
GENOMIC |
PCR |
SINGLE
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