Experiment information
Accession CRX028208
Organism Homo sapiens
Title CCP_64N
BioProject PRJCA000891
BioSample SAMC044199
Platform Ion Torrent PGM
Library
Library name Construction protocol Strategy Source Selection Layout
FFPE tissue was gravity collected into collection tubes. Paraffin is removed from FFPE LCM sections using Deparaffinization Solution (Qiagen) and DNA extraction is performed using AllPrep DNA/RNA FFPE kit (Qiagen) according to AllPrep DNA/RNA FFPE Handbook. Isolated genomic DNA concentration was verified using the Qubit dsDNA HS Assay Kit (Life Technologies, Carlsbad, California, USA). Samples were prepared as described in the Ion AmpliSeq Library Preparation User Guide, Publication #MAN0006735 Rev. 5.0 (Life Technologies) using the Ion AmpliSeq Library Kit 2.0 with Ion AmpliSeq Comprehensive Cancer Panel v2.0 (Life Technologies). AMPLICON GENOMIC PCR FRAGMENT
Processing Planned read length (bp): 150
Release date2019-12-31
Run
Run accession Release date Run data file information
File nameFile size (MB)
CRR031361 2019-12-31 CCP_64N.fastq.gz 525.85
SubmitterJin Gu (jgu@tsinghua.edu.cn)
OrganizationsTsinghua University
Date submitted2018-07-03
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