| Accession | CRX032317 |
| Organism | Oryza sativa |
| Title | Nip_mature_leaf_FAIRE_rep2 |
| BioProject | PRJCA000916 |
| BioSample | SAMC047859 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
~0.5 g of sample was cross-linked with 1% formaldehyde. We isolated nuclei and fragmented Chromatins into 200–600 bp with Bioruptor plus. After phenol-chloroform extraction and purification, DNA fragments of 200–400 bp were selected using AMPure XP beads (Beckman). FAIRE DNA was used for library preparation per instructions (NEBNext Ultra II DNA Library Prep Kit, New England Biolabs), and libraries were sequenced on the Illumina HiSeq X Ten platform. |
FAIRE-Seq |
GENOMIC |
size fractionation |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
| Release date | 2020-06-30 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR036193 |
CRR036193_f1.fq.gz
CRR036193_r2.fq.gz
|
1,600.5
1,885.98
|
|
| Submitter | Liang Xie (xieliang_bio@163.com) |
|---|
| Organization | Huazhong Agricultural University |
|---|
| Date submitted | 2018-11-03 |
|---|
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