| Accession | CRX032313 |
| Organism | Oryza sativa |
| Title | Nip_mature_leaf_H3K9me2_rep2 |
| BioProject | PRJCA000916 |
| BioSample | SAMC047855 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
~0.1 g of sample was used for ChIP-seq assay. Samples were fixed with formaldehyde under vacuum and stored at –80 °C until use. Chromatins were isolated with high effective method and fragmented into 200–600 bp with Bioruptor plus. ChIP assay was performed with H3K27me3 (ABconal, A2363). After reverse–cross–linking the protein-DNA complexes, immunoprecipitated DNA was purified by phenol:chloroform:isoamyl alcohol extraction. ChIP DNA was used for library preparation per instructions (NEBNext Ultra II DNA Library Prep Kit, New England Biolabs), and libraries were sequenced on the Illumina HiSeq X Ten platform. |
ChIP-Seq |
GENOMIC |
ChIP |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
| Release date | 2020-06-30 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR036189 |
CRR036189_f1.fq.gz
CRR036189_r2.fq.gz
|
861.23
1,049.57
|
|
| Submitter | Liang Xie (xieliang_bio@163.com) |
|---|
| Organization | Huazhong Agricultural University |
|---|
| Date submitted | 2018-11-03 |
|---|
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