Experiment information
Accession CRX027035
Organism Canis lupus familiaris
Title FAMIKEN00008.EXP1
BioProject PRJCA000879
BioSample SAMC036720
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
We used a QIAGEN DNeasy Blood & Tissue kit to extract genomic DNA and sheared to fragments randomly with the Covaris system. Then the libraries were constructed through end-repair, A-tailing, adapter ligation, and purification using the NEBNext Ultra DNA Library Prep Kit for Illumina. The concentrations of the libraries were measured using Qubit2.0. The libraries were diluted to 1.5ng/ul. Then the insert size was checked using Agilent 2100 and the purity was determined using Q-PCR method. Finally, it was sequenced on Illumina HiSeq platform. WGS GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
Release date2019-07-25
Run
Run accession Run data file information
File nameFile size (MB)
CRR030091 CRR030091_f1.fq.gz
CRR030091_r2.fq.gz
7,189.16
8,210.99
CRR030092 CRR030092_f1.fq.gz
CRR030092_r2.fq.gz
5,423.21
6,200.17
SubmitterBo-wen Zhou (zhoubowen@mail.kiz.ac.cn)
OrganizationKunming Institute of Zoology, Chinese Academy of Sciences
Date submitted2018-05-21