Experiment information
Accession CRX027033
Organism Canis lupus familiaris
Title FAMIKEN00003.EXP1
BioProject PRJCA000879
BioSample SAMC036718
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
We used a QIAGEN DNeasy Blood & Tissue kit to extract genomic DNA and sheared to fragments randomly with the Covaris system. Then the libraries were constructed through end-repair, A-tailing, adapter ligation, and purification using the NEBNext Ultra DNA Library Prep Kit for Illumina. The concentrations of the libraries were measured using Qubit2.0. The libraries were diluted to 1.5ng/ul. Then the insert size was checked using Agilent 2100 and the purity was determined using Q-PCR method. Finally, it was sequenced on Illumina HiSeq platform. WGS GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 275
Release date2019-07-25
Run
Run accession Run data file information
File nameFile size (MB)
CRR030087 CRR030087_f1.fq.gz
CRR030087_r2.fq.gz
3,692.22
3,982.62
CRR030088 CRR030088_f1.fq.gz
CRR030088_r2.fq.gz
3,960.15
4,233.29
CRR059191 CRR059191_f1.fq.gz
CRR059191_r2.fq.gz
3,516.69
3,789.54
SubmitterBo-wen Zhou (zhoubowen@mail.kiz.ac.cn)
OrganizationKunming Institute of Zoology, Chinese Academy of Sciences
Date submitted2018-05-21